CHEMPLOYMENT 796: Postdoctoral position at San Diego State University


Postdoctoral position at San Diego State University
Department of Chemistry and Biochemistry

We are looking for a highly motivated individual to start immediately in a NIH-funded postdoctoral position to study
structure and mechanisms of transfer-RNA modification enzymes using biochemical and X-ray crystallographic methods.
Prof. Manal Swairjo’s group focuses on structural, functional and mechanistic characterization of tRNA and DNA
modification enzymes and enzyme complexes, and related fundamental problems in molecular evolution and catalysis.
The laboratory is part of a long-standing multi-institutional consortium focused on studying the biosynthesis and
functions of the hypermodified nucleotides of transfer-RNA and understanding their roles in translation.

The postdoctoral position will focus on elucidating the molecular mechanisms underlying queuosine
modification of tRNA in eukaryotes using X-ray crystallography, biochemical and biophysical methods. The candidate will
be responsible for cloning, overexpression, purification, biochemical characterization, crystallization, and X-ray crystal
structure determination of protein and protein-RNA complexes, and mutagenesis toward structure-function analysis and
elucidation of enzyme mechanism. The researcher is expected to carry out experiments from molecular biology to a fully
refined structure, and complement structural data with biochemical and biophysical assays. The candidate will interface
with the PI and team of collaborators to complete functional and mechanistic interpretation of results.
The candidate should have a Ph.D. degree (in biochemistry, biophysics, structural biology or related area).
Experience with basic molecular biology, protein and RNA expression and purification, and strong oral and written
communication skills are required. Prior experience in macromolecular X-ray crystallography is desired. This is a full-time
position with competitive salary and fringe benefits.

For details and to apply, contact Manal Swairjo

SDSU is an equal opportunity employer and does not discriminate against persons on the basis of race, religion, national
origin, sexual orientation, gender, gender identity and expression, marital status, age, disability, pregnancy, medical
condition, or covered veteran status.